Composite

Part:BBa_K3757010:Design

Designed by: Erik Zimmer   Group: iGEM21_Tuebingen   (2021-09-28)


35S CtAEP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1882
    Illegal BamHI site found at 2138
    Illegal BamHI site found at 2309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66


Design Notes

Built by Golden Gate assembly; codon-optimized for expression in Nicotiana benthamiana; point mutations introduced in 35S promoter to remove BpiI restriction sites.


Source

CtAEP1 from Clitoria ternatea, sequence found through Genbank: KF918345.1 HA-tag originates from the the human influenza virus hemagglutinin protein, provided in Golden Gate vector. 35S promoter (BBa_K788000) and terminator (BBa_K1159307) originate from cauliflower mosaic virus, provided in Golden Gate vectors.

References